RNA Polymerases of Maize. Purification and Molecular Structure of DNA-dependent RNA Polymerase II

P. Mullinix, Kathleen; C. Strain, Gustave; Bogorad, Lawrence

doi:10.1073/pnas.70.8.2386

Public

RNA Polymerases of Maize. Purification and Molecular Structure of DNA-dependent RNA Polymerase II

Published in Proceedings of the National Academy of Sciences • Aug 1, 1973

Authors:

Kathleen P. Mullinix

Gustave C. Strain

Lawrence Bogorad

Abstract

Nuclear DNA-dependent RNA polymerase II has been purified from leaves of Zea mays by a new procedure that improves enzyme stability and thus permits more manipulation during purification. The purification procedure includes a heating step, gel filtration on Sepharose 6B and 4B, and chromatography on...

Subject

Polymerase

Gel electrophoresis

DNA

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