Lab in a tube: ultrasensitive detection of microRNAs at the single-cell level and in breast cancer patients using quadratic isothermal amplification.

R, Duan; X, Zuo; S, Wang; X, Quan; D, Chen; Z, Chen; L, Jiang; C, Fan; F, Xia

doi:10.1021/ja311313b

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Lab in a tube: ultrasensitive detection of microRNAs at the single-cell level and in breast cancer patients using quadratic isothermal amplification.

Shared by NobleBlocks on Mar 13, 2013 • 12:00 AM UTC

Authors:

Duan R

Zuo X

Wang S

Abstract

Through rational design of a functional molecular probe with high sequence specificity that takes advantage of sensitive isothermal amplification with simple operation, we developed a one-pot hairpin-mediated quadratic enzymatic amplification strategy for microRNA (miRNA) detection. Our method exhib...

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