Long-term culture of genome-stable bipotent stem cells from adult human liver.

Huch, Meritxell; Gehart, Helmuth; van Boxtel, Ruben; Hamer, Karien; Blokzijl, Francis; M A Verstegen, Monique; Ellis, Ewa; van Wenum, Martien; A Fuchs, Sabine; de Ligt, Joep; van de Wetering, Marc; Sasaki, Nobuo; J Boers, Susanne; Kemperman, Hans; de Jonge, Jeroen; N M Ijzermans, Jan; E S Nieuwenhuis, Edward; Hoekstra, Ruurdtje; Strom, Stephen; R G Vries, Robert; J W van der Laan, Luc; Cuppen, Edwin; Clevers, Hans

doi:10.1016/j.cell.2014.11.050

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Long-term culture of genome-stable bipotent stem cells from adult human liver.

Published in Cell • Jan 15, 2015

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Authors:

Meritxell Huch

,

Helmuth Gehart

,

Ruben van Boxtel

Abstract

Despite the enormous replication potential of the human liver, there are currently no culture systems available that sustain hepatocyte replication and/or function in vitro. We have shown previously that single mouse Lgr5+ liver stem cells can be expanded as epithelial organoids in vitro and can be ...

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