Improved flow cytometric detection of HLA alloantibodies using pronase: potential implications in renal transplantation.

S, Vaidya; TY, Cooper; J, Avandsalehi; T, Barnes; K, Brooks; P, Hymel; M, Noor; R, Sellers; A, Thomas; D, Stewart; J, Daller; JC, Fish; KK, Gugliuzza; RA, Bray

doi:10.1097/00007890-200102150-00015

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Improved flow cytometric detection of HLA alloantibodies using pronase: potential implications in renal transplantation.

Shared by NobleBlocks on Feb 1, 2001 • 12:00 AM UTC

Authors:

Vaidya S

Cooper TY

Avandsalehi J

Abstract

Background Flow cytomeric crossmatch (FCXM) has grown in popularity and has become the "standard of practice" in many programs. Although FCXM is the most sensitive method for detecting alloantibody, the B cell FCXM has been problematic. Difficulties with the B cell FCXMs have been centered around hi...

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