A Set of Vectors with a Tetracycline-Regulatable Promoter System for Modulated Gene Expression inSaccharomyces cerevisiae

Garí, Eloi; Piedrafita, Lídia; Aldea, Martí; Herrero, Enrique

doi:10.1002/(sici)1097-0061(199707)13:9<837::aid-yea145>3.0.co;2-t

Public

A Set of Vectors with a Tetracycline-Regulatable Promoter System for Modulated Gene Expression inSaccharomyces cerevisiae

Published in Yeast • Jul 1, 1997

Authors:

Eloi Garí

Lídia Piedrafita

Martí Aldea

Abstract

A set of Saccharomyces cerevisiae expression vectors has been developed in which transcription is driven by a hybrid tetO-CYC1 promoter through the action of a tetR-VP16 (tTA) activator. Expression from the promoter is regulated by tetracycline or derivatives. Various modalities of promoter and acti...

Subject

TetR

Biology

Activator (genetics)

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