A versatile and scalable two-step ion-exchange chromatography process for the purification of recombinant adeno-associated virus serotypes-2 and -5.

Brument, Nicole; Morenweiser, Robert; Blouin, Véronique; Toublanc, Estelle; Raimbaud, Isabelle; Chérel, Yan; Folliot, Sébastien; Gaden, Florence; Boulanger, Pierre; Kroner-Lux, Gabrielle; Moullier, Philippe; Rolling, Fabienne; Salvetti, Anna

doi:10.1006/mthe.2002.0719

Public

A versatile and scalable two-step ion-exchange chromatography process for the purification of recombinant adeno-associated virus serotypes-2 and -5.

Published in Molecular therapy : the journal of the American Society of Gene Therapy • Nov 1, 2002

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Authors:

Nicole Brument

,

Robert Morenweiser

,

Véronique Blouin

Abstract

Here we describe the development of a two-step chromatography process based on the use of ion-exchange resins for the purification of recombinant adeno-associated virus (rAAV) serotypes-2 and-5. In vitro and in vivo results demonstrate that this method, which does not require any prepurification ste...

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