Development and Validation of an Improved Oxygen Radical Absorbance Capacity Assay Using Fluorescein as the Fluorescent Probe

Ou, Boxin; Hampsch‐Woodill, Maureen; L. Prior, Ronald

doi:10.1021/jf010586o

Public

Development and Validation of an Improved Oxygen Radical Absorbance Capacity Assay Using Fluorescein as the Fluorescent Probe

Published in Journal of Agricultural and Food Chemistry • Sep 18, 2001

Authors:

Boxin Ou

Maureen Hampsch‐Woodill

Ronald L. Prior

Abstract

An improved method of oxygen radical absorbance capacity (ORAC) assay has been developed and validated using fluorescein (3',6'-dihydroxyspiro[isobenzofuran-1[3H],9'[9H]-xanthen]-3-one) as the fluorescent probe. Our results demonstrate that fluorescein (FL) is superior to B-phycoerythrin. The oxidiz...

Subject

Oxygen radical absorbance capacity

Chemistry

Fluorescein

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