The Positive Effect of the Decreased NADPH-Preferring Activity of Xylose Reductase from<i>Pichia stipitis</i>on Ethanol Production Using Xylose-Fermenting Recombinant<i>Saccharomyces cerevisiae</i>

Watanabe, Seiya; Pil Pack, Seung; Abu Saleh, Ahmed; Annaluru, Narayana; Kodaki, Tsutomu; Makino, Keisuke

doi:10.1271/bbb.70104

Public

The Positive Effect of the Decreased NADPH-Preferring Activity of Xylose Reductase fromPichia stipitison Ethanol Production Using Xylose-Fermenting RecombinantSaccharomyces cerevisiae

Published in Bioscience Biotechnology and Biochemistry • May 23, 2007

Authors:

Seiya Watanabe

Seung Pil Pack

Ahmed Abu Saleh

Abstract

We focused on the effects of a mutation of xylose reductase from Pichia stipitis (PsXR) on xylose-to-ethanol fermentation using recombinant Saccharomyces cerevisiae transformed with PsXR and PsXDH (xylitol dehydrogenase from P. stipitis) genes. Based on inherent NADH-preferring XR and several site-d...

Subject

Pichia stipitis

Xylose

Xylitol

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