An In Vivo Fluorescent Sensor Reveals Intracellular Ins(1,3,4,5)P<sub>4</sub>Dynamics in Single Cells

Sakaguchi, Reiko; Tainaka, Kazuki; Shimada, Naoko; Nakano, Shun; Inoue, Masafumi; Kiyonaka, Shigeki; Mori, Yasuo; Morii, Takashi

doi:10.1002/anie.200903951

Public

An In Vivo Fluorescent Sensor Reveals Intracellular Ins(1,3,4,5)P4Dynamics in Single Cells

Published in Angewandte Chemie International Edition • Nov 8, 2009

Authors:

Reiko Sakaguchi

Kazuki Tainaka

Naoko Shimada

Abstract

Don′t shoot the messenger! An optical sensor for D-myo-inositol 1,3,4,5-tetrakisphosphate (Ins(1,3,4,5)P4), an intracellular second messenger, is constructed by introducing a fluorophore at a unique cysteine residue within a mutant of the pleckstrin homology (PH) domain of the general receptor for p...

Subject

Second messenger system

Pleckstrin homology domain

Intracellular

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