Genome-Wide Insertional Mutagenesis of <i>Arabidopsis thaliana</i>

M. Alonso, José; N. Stepanova, Anna; J. Leisse, Thomas; J. Kim, Christopher; Chen, Huaming; Shinn, Paul; K. Stevenson, Denise; Zimmerman, Justin; Barajas, Pascual; Cheuk, Rosa; Gadrinab, Carmelita; Heller, Collen; Jeske, Albert; Koesema, Eric; C. Meyers, Cristina; Parker, Holly; Prednis, Lance; Ansari, Yasser; Choy, Nathan; Deen, Hashim; Geralt, M.; Hazari, Nisha; Hom, Emily; Karnes, Meagan; B. Mulholland, Celene; Ndubaku, Ral; Schmidt, Ian; Guzmán, Plinio; Aguilar‐Henonin, Laura; Schmid, Markus; Weigel, Detlef; E. Carter, David; Marchand, Trudy; Risseeuw, Eddy; Brogden, Debra; Zeko, Albana; L. Crosby, William; C. Berry, Charles; R. Ecker, Joseph

doi:10.1126/science.1086391

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Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana

Published in Science • Aug 1, 2003

Authors:

José M. Alonso

Anna N. Stepanova

Thomas J. Leisse

Abstract

Over 225,000 independent Agrobacterium transferred DNA (T-DNA) insertion events in the genome of the reference plant Arabidopsis thaliana have been created that represent near saturation of the gene space. The precise locations were determined for more than 88,000 T-DNA insertions, which resulted in...

Subject

Insertional mutagenesis

Arabidopsis thaliana

Genetics

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