Selective fluorescent imaging of superoxide
                    <i>in vivo</i>
                    using ethidium-based probes

M. Robinson, Kristine; S. Janes, Michael; Pehar, Mariana; S. Monette, Jeffrey; F. Ross, Meredith; M. Hagen, Tory; P. Murphy, Michael; S. Beckman, Joseph

doi:10.1073/pnas.0601945103

Public

Selective fluorescent imaging of superoxide in vivo using ethidium-based probes

Shared by NobleBlocks on Oct 10, 2006 • 12:00 AM UTC

Authors:

Kristine M. Robinson

Michael S. Janes

Mariana Pehar

Abstract

The putative oxidation of hydroethidine (HE) has become a widely used fluorescent assay for the detection of superoxide in cultured cells. By covalently joining HE to a hexyl triphenylphosphonium cation (Mito-HE), the HE moiety can be targeted to mitochondria. However, the specificity of HE and Mito...

Discussions