Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants

P. Hellens, Roger; C. Allan, Andrew; N. Friel, Ellen; Bolitho, Karen; Grafton, Karryn; D. Templeton, Matthew; Karunairetnam, Sakuntala; P. Gleave, Andrew; A. Laing, William

doi:10.1186/1746-4811-1-13

Public

Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants

Published in Plant Methods • Dec 1, 2005

Authors:

Roger P. Hellens

Andrew C. Allan

Ellen N. Friel

Abstract

BACKGROUND: We describe novel plasmid vectors for transient gene expression using Agrobacterium, infiltrated into Nicotiana benthamiana leaves. We have generated a series of pGreenII cloning vectors that are ideally suited to transient gene expression, by removing elements of conventional binary vec...

Subject

Biology

Multiple cloning site

Functional genomics

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