Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients

Heuer, Holger; Kršek, Michal; Baker, Paul; Smalla, Kornelia; M. H. Wellington, Elizabeth

doi:10.1128/aem.63.8.3233-3241.1997

Public

Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients

Published in Applied and Environmental Microbiology • Aug 1, 1997

Authors:

Holger Heuer

Michal Kršek

Paul Baker

Abstract

A group-specific primer, F243 (positions 226 to 243, Escherichia coli numbering), was developed by comparison of sequences of genes encoding 16S rRNA (16S rDNA) for the detection of actinomycetes in the environment with PCR and temperature or denaturing gradient gel electrophoresis (TGGE or DGGE, re...

Subject

Temperature gradient gel electrophoresis

Biology

16S ribosomal RNA

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