MAGeCK enables robust identification of essential genes from genome-scale CRISPR/Cas9 knockout screens

Li, Wei; Xu, Han; Xiao, Tengfei; Cong, Le; I. Love, Michael; Zhang, Feng; A. Irizarry, Rafael; S. Liu, Jun; Brown, Myles; Shirley Liu, X.

doi:10.1186/s13059-014-0554-4

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MAGeCK enables robust identification of essential genes from genome-scale CRISPR/Cas9 knockout screens

Published in Genome biology • Dec 4, 2014

Authors:

Wei Li

Han Xu

Tengfei Xiao

Abstract

We propose the Model-based Analysis of Genome-wide CRISPR/Cas9 Knockout (MAGeCK) method for prioritizing single-guide RNAs, genes and pathways in genome-scale CRISPR/Cas9 knockout screens. MAGeCK demonstrates better performance compared with existing methods, identifies both positively and negativel...

Subject

CRISPR

Biology

Human genetics

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