Development and evaluation of a multiplex PCR assay for rapid detection of toxigenic<i>Vibrio cholerae</i>O1 and O139

Hoshino, Katsuaki; Yamasaki, S; K. Mukhopadhyay, Asish; Chakraborty, Soumen; Basu, Arnab; Bhattacharya, Sujit; Balakrish Nair, G.; Shimada, Toshio; Takeda, Yoshifumi

doi:10.1111/j.1574-695x.1998.tb01128.x

Public

Development and evaluation of a multiplex PCR assay for rapid detection of toxigenicVibrio choleraeO1 and O139

Published in FEMS Immunology & Medical Microbiology • Mar 1, 1998

Authors:

Katsuaki Hoshino

S Yamasaki

Asish K. Mukhopadhyay

Abstract

A multiplex polymerase chain reaction assay was developed for concurrent detection of rfb sequences specific for the O1 and the O139 serogroups of Vibrio cholerae and for ctxA specific sequences. The multiplex PCR assay was found to be highly specific and sensitive and was capable of detecting 65 cf...

Subject

Vibrio cholerae

Multiplex polymerase chain reaction

Biology

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