Development and Evaluation of a Novel Loop-Mediated Isothermal Amplification Method for Rapid Detection of Severe Acute Respiratory Syndrome Coronavirus

Thai, Hong; Quynh Le, Mai; Duc Vuong, Cuong; Parida, Manmohan; Minekawa, Harumi; Notomi, Tsugunori; Hasebe, Futoshi; Morita, Kouichi

doi:10.1128/jcm.42.5.1956-1961.2004

Public

Development and Evaluation of a Novel Loop-Mediated Isothermal Amplification Method for Rapid Detection of Severe Acute Respiratory Syndrome Coronavirus

Published in Journal of Clinical Microbiology • May 1, 2004

Authors:

Hong Thai

Mai Quynh Le

Cuong Duc Vuong

Abstract

The development and evaluation of a one-step single-tube accelerated real-time quantitative reverse transcription (RT) loop-mediated isothermal amplification (LAMP) assay is reported for rapid detection of the severe acute respiratory syndrome coronavirus (SARS-CoV) replicase gene. A total of 49 sam...

Subject

Loop-mediated isothermal amplification

Reverse Transcription Loop-mediated Isothermal Amplification

Virology

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