Development and evaluation of a novel loop-mediated isothermal amplification method for rapid detection of severe acute respiratory syndrome coronavirus.

TC, Hong; QL, Mai; DV, Cuong; M, Parida; H, Minekawa; T, Notomi; F, Hasebe; K, Morita

doi:10.1128/jcm.42.5.1956-1961.2004

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Development and evaluation of a novel loop-mediated isothermal amplification method for rapid detection of severe acute respiratory syndrome coronavirus.

Shared by NobleBlocks on May 1, 2004 • 12:00 AM UTC

Authors:

Hong TC

Mai QL

Cuong DV

Abstract

The development and evaluation of a one-step single-tube accelerated real-time quantitative reverse transcription (RT) loop-mediated isothermal amplification (LAMP) assay is reported for rapid detection of the severe acute respiratory syndrome coronavirus (SARS-CoV) replicase gene. A total of 49 sam...

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