Function-based gene identification using enzymatically generated normalized shRNA library and massive parallel sequencing.

Shtutman, Michael; Maliyekkel, Anil; Shao, Yu; Steven Carmack, C; Baig, Mirza; Warholic, Natalie; Cole, Kelly; V Broude, Eugenia; T Harkins, Timothy; Ding, Ye; B Roninson, Igor

doi:10.1073/pnas.1003055107

Public

Function-based gene identification using enzymatically generated normalized shRNA library and massive parallel sequencing.

Published in Proceedings of the National Academy of Sciences of the United States of America • Apr 20, 2010

NobleIDark:/48914/w1/20260526/EC31D0A3Record on NobleID

Authors:

Michael Shtutman

,

Anil Maliyekkel

,

Yu Shao

Abstract

As a general strategy for function-based gene identification, an shRNA library containing approximately 150 shRNAs per gene was enzymatically generated from normalized (reduced-redundance) human cDNA. The library was constructed in an inducible lentiviral vector, enabling propagation of growth-inhib...

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