Comprehensive Identification of Cell Cycle–regulated Genes of the Yeast<i>Saccharomyces cerevisiae</i>by Microarray Hybridization

T. Spellman, Paul; Sherlock, Gavin; Q. Zhang, Michael; R. Iyer, Vishwanath; Anders, Kirk; B. Eisen, Michael; O. Brown, Patrick; Botstein, David; Futcher, Bruce

doi:10.1091/mbc.9.12.3273

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Comprehensive Identification of Cell Cycle–regulated Genes of the YeastSaccharomyces cerevisiaeby Microarray Hybridization

Shared by NobleBlocks on Dec 1, 1998 • 12:00 AM UTC

Authors:

Paul T. Spellman

Gavin Sherlock

Michael Q. Zhang

Abstract

We sought to create a comprehensive catalog of yeast genes whose transcript levels vary periodically within the cell cycle. To this end, we used DNA microarrays and samples from yeast cultures synchronized by three independent methods: α factor arrest, elutriation, and arrest of a cdc15 temperature-...

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