Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators

Tian, Lin; Andrew Hires, Samuel; Mao, Tianyi; Huber, Daniel; Eugenia Chiappe, M; H. Chalasani, Sreekanth; Petreanu, Leopoldo; Akerboom, Jasper; McKinney, Sean; R. Schreiter, Eric; I. Bargmann, Cornelia; Jayaraman, Vivek; Svoboda, Karel; L. Looger, Loren

doi:10.1038/nmeth.1398

Public

Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators

Published in Nature Methods • Nov 8, 2009

Authors:

Lin Tian

Samuel Andrew Hires

Tianyi Mao

Abstract

Genetically encoded calcium indicators (GECIs) can be used to image activity in defined neuronal populations. However, current GECIs produce inferior signals compared to synthetic indicators and recording electrodes, precluding detection of low firing rates. We developed a single-wavelength GCaMP2-b...

Subject

Calcium imaging

Calcium

Neuroscience

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