Evaluation and Improvement of Real-Time PCR Assays Targeting lytA , ply , and psaA Genes for Detection of Pneumococcal DNA

da Glória S. Carvalho, Maria; Lucia Tondella, M.; A. McCaustland, Karen; Weidlich, Luciana; McGee, Lesley; W. Mayer, Leonard; G. Steigerwalt, Arnold; Whaley, Melissa; R. Facklam, Richard; S. Fields, Barry; M. Carlone, George; W. Ades, Edwin; Dagan, Ron; S. Sampson, Jacquelyn

doi:10.1128/jcm.02498-06

Public

Evaluation and Improvement of Real-Time PCR Assays Targeting lytA , ply , and psaA Genes for Detection of Pneumococcal DNA

Published in Journal of Clinical Microbiology • May 31, 2007

Authors:

Maria da Glória S. Carvalho

M. Lucia Tondella

Karen A. McCaustland

Abstract

The accurate diagnosis of pneumococcal disease has frequently been hampered not only by the difficulties in obtaining isolates of the organism from patient specimens but also by the misidentification of pneumococcus-like viridans group streptococci (P-LVS) as Streptococcus pneumoniae. This is especi...

Subject

Streptococcus pneumoniae

Microbiology

Biology

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