High‐accuracy <i>de novo</i> assembly and <scp>SNP</scp> detection of chloroplast genomes using a <scp>SMRT</scp> circular consensus sequencing strategy

Li, Qiushi; Li, Ying; Song, Jingyuan; Xu, Haibin; Xu, Jiang; Zhu, Yingjie; Li, Xiwen; Gao, Huanhuan; Dong, Linlin; Qian, Jun; Sun, Chao; Chen, Shilin

doi:10.1111/nph.12966

Public

High‐accuracy de novo assembly and SNP detection of chloroplast genomes using a SMRT circular consensus sequencing strategy

Published in New Phytologist • Aug 8, 2014

Authors:

Qiushi Li

Ying Li

Jingyuan Song

Abstract

A circular consensus sequencing (CCS) strategy involving single molecule, real-time (SMRT) DNA sequencing technology was applied to de novo assembly and single nucleotide polymorphism (SNP) detection of chloroplast genomes. Chloroplast DNA was purified from enriched chloroplasts of pooled individual...

Subject

Genome

Biology

Sequence assembly

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