Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA

Muyzer, Gerard; C. de Waal, Ellen; G. Uitterlinden, André

doi:10.1128/aem.59.3.695-700.1993

Public

Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA

Published in Applied and Environmental Microbiology • Mar 1, 1993

Authors:

Gerard Muyzer

Ellen C. de Waal

André G. Uitterlinden

Abstract

We describe a new molecular approach to analyzing the genetic diversity of complex microbial populations. This technique is based on the separation of polymerase chain reaction-amplified fragments of genes coding for 16S rRNA, all the same length, by denaturing gradient gel electrophoresis (DGGE). D...

Subject

Temperature gradient gel electrophoresis

Biology

Polymerase chain reaction

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