Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators

Imamura, Hiromi; Phuong Huynh Nhat, Kim; Togawa, Hiroko; Saito, Kenta; Iino, Ryota; Kato‐Yamada, Yasuyuki; Nagai, Takeharu; Noji, Hiroyuki

doi:10.1073/pnas.0904764106

Public

Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators

Published in Proceedings of the National Academy of Sciences • Aug 31, 2009

Authors:

Hiromi Imamura

Kim Phuong Huynh Nhat

Hiroko Togawa

Abstract

Adenosine 5'-triphosphate (ATP) is the major energy currency of cells and is involved in many cellular processes. However, there is no method for real-time monitoring of ATP levels inside individual living cells. To visualize ATP levels, we generated a series of fluorescence resonance energy transfe...

Subject

ATP synthase

Oligomycin

V-ATPase

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