Development of a Real-Time Reverse Transcriptase PCR Assay for Type A Influenza Virus and the Avian H5 and H7 Hemagglutinin Subtypes

Spackman, Erica; A. Senne, Dennis; J. Myers, Thomas; L. Bulaga, Leslie; Garber, Lindsey; L. Perdue, Michael; L. Lohman, Kenton; T. Daum, Luke; L. Suarez, David

doi:10.1128/jcm.40.9.3256-3260.2002

Public

Development of a Real-Time Reverse Transcriptase PCR Assay for Type A Influenza Virus and the Avian H5 and H7 Hemagglutinin Subtypes

Published in Journal of Clinical Microbiology • Aug 29, 2002

Authors:

Erica Spackman

Dennis A. Senne

Thomas J. Myers

Abstract

A real-time reverse transcriptase PCR (RRT-PCR) assay based on the avian influenza virus matrix gene was developed for the rapid detection of type A influenza virus. Additionally, H5 and H7 hemagglutinin subtype-specific probe sets were developed based on North American avian influenza virus sequenc...

Subject

Virology

Hemagglutinin (influenza)

Biology

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