Rapid detection of carbapenemase genes by multiplex real-time PCR.
Published in The Journal of antimicrobial chemotherapy • Apr 1, 2012
Authors:,,
Jussimara Monteiro
Raymond H Widen
Antonio C C Pignatari
Abstract
OBJECTIVES: To develop a single multiplex real-time PCR assay to detect six different genetic types of carbapenemases already identified in Enterobacteriaceae (KPC, GES, NDM, IMP, VIM and OXA-48). METHODS: A total of 58 bacterial isolates were tested. Thirty were previously characterized as resistan...
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