Identification of Mycobacterial Species by Comparative Sequence Analysis of the RNA Polymerase Gene ( <i>rpoB</i> )

Kim, Bum‐Joon; Hyun Lee, Seung; Lyu, Mi-Ae; Kim, Seo-Jeong; Bai, Gill‐Han; Kim, Sang‐Jae; Chae, Gue‐Tae; Kim, Eui-Chong; Cha, Chang-Yong; Kook, Yoon‐Hoh

doi:10.1128/jcm.37.6.1714-1720.1999

Public

Identification of Mycobacterial Species by Comparative Sequence Analysis of the RNA Polymerase Gene ( rpoB )

Published in Journal of Clinical Microbiology • Jun 1, 1999

Authors:

Bum‐Joon Kim

Seung Hyun Lee

Mi-Ae Lyu

Abstract

For the differentiation and identification of mycobacterial species, the rpoB gene, encoding the beta subunit of RNA polymerase, was investigated. rpoB DNAs (342 bp) were amplified from 44 reference strains of mycobacteria and clinical isolates (107 strains) by PCR. The nucleotide sequences were dir...

Subject

rpoB

Biology

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