Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies

Klindworth, Anna; Pruesse, Elmar; Schweer, Timmy; Peplies, Jörg; Quast, Christian; Horn, Matthias; Oliver Glöckner, Frank

doi:10.1093/nar/gks808

Public

Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies

Published in Nucleic Acids Research • Aug 28, 2012

Authors:

Anna Klindworth

Elmar Pruesse

Timmy Schweer

Abstract

16S ribosomal RNA gene (rDNA) amplicon analysis remains the standard approach for the cultivation-independent investigation of microbial diversity. The accuracy of these analyses depends strongly on the choice of primers. The overall coverage and phylum spectrum of 175 primers and 512 primer pairs w...

Subject

Biology

Amplicon

16S ribosomal RNA

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