One-step inactivation of chromosomal genes in
                    <i>Escherichia coli</i>
                    K-12 using PCR products

A. Datsenko, Kirill; L. Wanner, Barry

doi:10.1073/pnas.120163297

Public

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products

Shared by NobleBlocks on May 28, 2000 • 12:00 AM UTC

Authors:

Kirill A. Datsenko

Barry L. Wanner

Abstract

We have developed a simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology to the targeted gene(s). In this procedure, recombination requires the phage λ Red recombinase, which is synthesized under the control of an inducible pro...

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