Development of a Dual-Index Sequencing Strategy and Curation Pipeline for Analyzing Amplicon Sequence Data on the MiSeq Illumina Sequencing Platform

J. Kozich, James; L. Westcott, Sarah; T. Baxter, Nielson; K. Highlander, Sarah; D. Schloss, Patrick

doi:10.1128/aem.01043-13

Public

Development of a Dual-Index Sequencing Strategy and Curation Pipeline for Analyzing Amplicon Sequence Data on the MiSeq Illumina Sequencing Platform

Published in Applied and Environmental Microbiology • Jun 21, 2013

Authors:

James J. Kozich

Sarah L. Westcott

Nielson T. Baxter

Abstract

Rapid advances in sequencing technology have changed the experimental landscape of microbial ecology. In the last 10 years, the field has moved from sequencing hundreds of 16S rRNA gene fragments per study using clone libraries to the sequencing of millions of fragments per study using next-generati...

Subject

Metagenomics

Shotgun sequencing

Illumina dye sequencing

Research Assistant

AI chat, annotations, notes & similar papers

Finding related papers...

Discussions

(0)

No comments yet

Be the first to share your thoughts!