Generation of Human Monoclonal Antibodies against HIV-1 Proteins; Electrofusion and Epstein-Barr Virus Transformation for Peripheral Blood Lymphocyte Immortalization

Buchacher, Andrea; PREDL, RENATE; Strutzenberger, K.; Steinfellner, W.; Trkola, Alexandra; Purtscher, Martin; Grüber, Gerhard; Tauer, Christa; Steindl, Franz; Jungbauer, Alois; Katinger, H.

doi:10.1089/aid.1994.10.359

Public

Generation of Human Monoclonal Antibodies against HIV-1 Proteins; Electrofusion and Epstein-Barr Virus Transformation for Peripheral Blood Lymphocyte Immortalization

Published in AIDS Research and Human Retroviruses • Apr 1, 1994

Authors:

Andrea Buchacher

RENATE PREDL

K. Strutzenberger

Abstract

Electrofusion and EBV transformation were studied by immortalizing human PBLs from blood of HIV-1-positive volunteers. A panel of 33 cell lines producing human monoclonal antibodies (Hu-MAbs) against HIV-1 was established by cell fusion or EBV transformation. For the first fusion experiments the sou...

Subject

Monoclonal antibody

Virology

Electrofusion

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