DNA Extraction from Soils: Old Bias for New Microbial Diversity Analysis Methods

Martin‐Laurent, Fabrice; Philippot, Laurent; Hallet, S.; Chaussod, R.; C. Germon, J.; Soulas, Guy; Catroux, G.

doi:10.1128/aem.67.5.2354-2359.2001

Public

DNA Extraction from Soils: Old Bias for New Microbial Diversity Analysis Methods

Published in Applied and Environmental Microbiology • May 1, 2001

Authors:

Fabrice Martin‐Laurent

Laurent Philippot

S. Hallet

Abstract

The impact of three different soil DNA extraction methods on bacterial diversity was evaluated using PCR-based 16S ribosomal DNA analysis. DNA extracted directly from three soils showing contrasting physicochemical properties was subjected to amplified ribosomal DNA restriction analysis and ribosoma...

Subject

Ribosomal Intergenic Spacer analysis

Phylotype

DNA extraction

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