Multiplexed Protein Quantitation in Saccharomyces cerevisiae Using Amine-reactive Isobaric Tagging Reagents

L. Ross, Philip; Huang, Yulin; Marchese, Jason; Williamson, B.; C. Parker, Kenneth; J. Hattan, Stephen; Khainovski, Nikita; Pillai, Sasi; Dey, Subhakar; B. Daniels, Scott; Purkayastha, Subhasish; Juhász, Péter; A. Martin, Stephen; Bartlet‐Jones, Michael; He, Feng; Jacobson, Allan; Pappin, Darryl

doi:10.1074/mcp.m400129-mcp200

Public

Multiplexed Protein Quantitation in Saccharomyces cerevisiae Using Amine-reactive Isobaric Tagging Reagents

Published in Molecular & Cellular Proteomics • Sep 22, 2004

Authors:

Philip L. Ross

Yulin Huang

Jason Marchese

Abstract

We describe here a multiplexed protein quantitation strategy that provides relative and absolute measurements of proteins in complex mixtures. At the core of this methodology is a multiplexed set of isobaric reagents that yield amine-derivatized peptides. The derivatized peptides are indistinguishab...

Subject

Isobaric labeling

Chemistry

Isobaric process

Research Assistant

AI chat, annotations, notes & similar papers

Finding related papers...

Discussions

(0)

No comments yet

Be the first to share your thoughts!