Simultaneous Detection of <i>Neisseria meningitidis, Haemophilus influenzae,</i> and <i>Streptococcus pneumoniae</i> in Suspected Cases of Meningitis and Septicemia Using Real-Time PCR

Corless, Caroline; Guiver, Malcolm; Borrow, Ray; Edwards-Jones, V.; Fox, Áine; B. Kaczmarski, Edward

doi:10.1128/jcm.39.4.1553-1558.2001

Public

Simultaneous Detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in Suspected Cases of Meningitis and Septicemia Using Real-Time PCR

Published in Journal of Clinical Microbiology • Apr 1, 2001

Authors:

Caroline Corless

Malcolm Guiver

Ray Borrow

Abstract

A single-tube 5' nuclease multiplex PCR assay was developed on the ABI 7700 Sequence Detection System (TaqMan) for the detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae from clinical samples of cerebrospinal fluid (CSF), plasma, serum, and whole blood. Capsula...

Subject

Neisseria meningitidis

Streptococcus pneumoniae

Haemophilus influenzae

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