Mechanisms of in Vivo DNA Electrotransfer: Respective Contributions of Cell Electropermeabilization and DNA Electrophoresis

Šatkauskas, Saulius; F. Bureau, Michel; Puc, Marko; Mahfoudi, Abderrahim; Scherman, Daniel; Miklavčič, Damijan; M. Mir, Lluis

doi:10.1006/mthe.2002.0526

Public

Mechanisms of in Vivo DNA Electrotransfer: Respective Contributions of Cell Electropermeabilization and DNA Electrophoresis

Published in Molecular Therapy • Feb 1, 2002

Authors:

Saulius Šatkauskas

Michel F. Bureau

Marko Puc

Abstract

Efficient cell electrotransfection can be achieved using combinations of high-voltage (HV; 800 V/cm, 100 micros) and low-voltage (LV; 80 V/cm, 100 ms) pulses. We have developed equipment allowing the generation of various HV and LV combinations with precise control of the lag between the HV and LV p...

Subject

Electroporation

DNA

Pulse (music)

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