Total Synthesis of a Functional Designer Eukaryotic Chromosome

Annaluru, Narayana; Muller, Héloïse; A. Mitchell, Leslie; Ramalingam, Sivaprakash; Stracquadanio, Giovanni; M. Richardson, Sarah; S. Dymond, Jessica; Kuang, Zheng; Z. Scheifele, Lisa; M. Cooper, Eric; Cai, Yizhi; Zeller, Karen; Agmon, Neta; S. Han, Jeffrey; Hadjithomas, Michalis; Tullman, Jennifer; Caravelli, Katrina; M. Cirelli, Kimberly; Guo, Zheyuan; London, Viktoriya; Yeluru, Apurva; Murugan, Sindurathy; Kandavelou, Karthikeyan; Agier, Nicolas; Fischer, Gilles; Yang, Kun; A. Martin, James; Bilgel, Murat; Bohutskyi, Pavlo; M. Boulier, Kristin; J. Capaldo, Brian; W. Chang, Joy; Charoen, Kristie; Jin Choi, Woo; Deng, Peter; E. DiCarlo, James; Y. H. Doong, J.; Dunn, Jessilyn; I. Feinberg, Jason; Fernandez, Christopher; E. Floria, Charlotte; Gladowski, David; Hadidi, Pasha; E. Ishizuka, Isabel; Jabbari, Javaneh; Y. L. Lau, Calvin; A. Lee, Pablo; Li, Sean; Lin, Denise; E. Linder, Matthias; P. Ling, Jonathan; Liu, Jaime; Liu, Jonathan; London, Mariya; Ma, Henry; Mao, Jessica; E. McDade, Jessica; McMillan, Alexandra; M. Moore, Aaron; Chan Oh, Won; Ouyang, Yu; Patel, Ruchi; Paul, Marina; C. Paulsen, Laura; Qiu, Judy; Rhee, Alex; G. Rubashkin, Matthew; Y. Soh, Ina; E. Sotuyo, Nathaniel; Srinivas, Venkatesh; Suarez, Allison; O.-T. Wong, Andy; Wong, Remus; Xie, Wei; Xu, Yijie; T. Yu, Allen; Koszul, Romain; S. Bader, Joel; D. Boeke, Jef; Chandrasegaran, Srinivasan

doi:10.1126/science.1249252

Public

Total Synthesis of a Functional Designer Eukaryotic Chromosome

Published in Science • Mar 28, 2014

Authors:

Narayana Annaluru

Héloïse Muller

Leslie A. Mitchell

Abstract

Rapid advances in DNA synthesis techniques have made it possible to engineer viruses, biochemical pathways and assemble bacterial genomes. Here, we report the synthesis of a functional 272,871-base pair designer eukaryotic chromosome, synIII, which is based on the 316,617-base pair native Saccharomy...

Subject

Chromosome

Biology

Functional design

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