Plasma leptin determination in ruminants: effect of nutritional status and body fatness on plasma leptin concentration assessed by a specific RIA in sheep

Delavaud, Carole; Bocquier, François; Chilliard, Yves; H. Keisler, D.; Gertler, Arieh; Kann, G.

doi:10.1677/joe.0.1650519

Public

Plasma leptin determination in ruminants: effect of nutritional status and body fatness on plasma leptin concentration assessed by a specific RIA in sheep

Published in Journal of Endocrinology • May 1, 2000

Authors:

Carole Delavaud

François Bocquier

Yves Chilliard

Abstract

A specific leptin RIA was developed to assess concentrations of leptin in ovine plasma, and was shown to be efficient with bovine and caprine plasma. A specific, high-affinity antibody was generated against recombinant ovine leptin which, when used in a competitive leptin RIA, provided valid estimat...

Subject

Leptin

Internal medicine

Endocrinology

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