Validation and Application of a PCR Primer Set to Quantify Fungal Communities in the Soil Environment by Real-Time Quantitative PCR

Chemidlin Prévost‐Bouré, Nicolas; Christen, Richard; Dequiedt, Samuel; Mougel, Christophe; Lelièvre, Mélanie; Jolivet, Claudy; Reza Shahbazkia, Hamid; Guillou, Laure; Arrouays, Dominique; Ranjard, Lionel

doi:10.1371/journal.pone.0024166

Public

Validation and Application of a PCR Primer Set to Quantify Fungal Communities in the Soil Environment by Real-Time Quantitative PCR

Published in PLoS ONE • Sep 8, 2011

Authors:

Nicolas Chemidlin Prévost‐Bouré

Richard Christen

Samuel Dequiedt

Abstract

Fungi constitute an important group in soil biological diversity and functioning. However, characterization and knowledge of fungal communities is hampered because few primer sets are available to quantify fungal abundance by real-time quantitative PCR (real-time Q-PCR). The aim in this study was to...

Subject

Amplicon

Primer (cosmetics)

In silico

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