Fertilization in Vitro and Development of Mouse Ova

Epididymal sperm were added to embryological watch glasses containing 396 F1, hybrid mouse ova in a simple medium under paraffin oil. The dishes were gently agitated for 8 h in an atmosphere of 5% CO2, 5% O2, and 90% N2. The ova were washed and cultured in glass tubes for 96 h during which time 365 ... View More