Highly Sensitive Determination of microRNA Using Target‐Primed and Branched Rolling‐Circle Amplification

Cheng, Yongqiang; Zhang, Xian; Li, Zhengping; Jiao, Xiaoxia; Wang, Yucong; Zhang, Yali

doi:10.1002/anie.200805665

Public

Highly Sensitive Determination of microRNA Using Target‐Primed and Branched Rolling‐Circle Amplification

Published in Angewandte Chemie International Edition • Feb 13, 2009

Authors:

Yongqiang Cheng

Xian Zhang

Zhengping Li

Abstract

One-nucleotide differences in microRNAs (miRNAs) can be discriminated in an assay based on a branched rolling-circle amplification (BRCA) reaction and fluorescence quantification. With the proposed method miRNA can be detected at concentrations as low as 10 fM, and the miRNA in a total RNA sample of...

Subject

microRNA

Rolling circle replication

Nucleotide

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