Simultaneous Detection and Quantification of Ancylostoma duodenale, Necator americanus, and Oesophagostomum bifurcum in Fecal Samples Using Multiplex Real-Time PCR

J. Verweij, Jaco; A. T. Brienen, Eric; B. Ziem, Juventus; Yelifari, Lawrence; M. Polderman, Anton; van Lieshout, Lisette

doi:10.4269/ajtmh.2007.77.685

Public

Simultaneous Detection and Quantification of Ancylostoma duodenale, Necator americanus, and Oesophagostomum bifurcum in Fecal Samples Using Multiplex Real-Time PCR

Published in American Journal of Tropical Medicine and Hygiene • Oct 1, 2007

Authors:

Jaco J. Verweij

Eric A. T. Brienen

Juventus B. Ziem

Abstract

A multiplex real-time PCR was developed and evaluated for the simultaneous detection of Ancylostoma duodenale, Necator americanus, and Oesophagostomum bifurcum in fecal samples. Using well-defined control samples (N = 150), known positive fecal samples (N = 50), and fecal samples from an area in Gha...

Subject

Necator americanus

Ancylostoma duodenale

Feces

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