Diagnostic Real-Time PCR for Detection of <i>Salmonella</i> in Food

Malorny, Burkhard; Paccassoni, Elisa; Fach, Patrick; Bunge, Cornelia; Martin, Annett; Helmuth, Reiner

doi:10.1128/aem.70.12.7046-7052.2004

Public

Diagnostic Real-Time PCR for Detection of Salmonella in Food

Published in Applied and Environmental Microbiology • Dec 1, 2004

Authors:

Burkhard Malorny

Elisa Paccassoni

Patrick Fach

Abstract

A robust 5' nuclease (TaqMan) real-time PCR was developed and validated in-house for the specific detection of Salmonella in food. The assay used specifically designed primers and a probe target within the ttrRSBCA locus, which is located near the Salmonella pathogenicity island 2 at centisome 30.5....

Subject

Salmonella

TaqMan

Biology

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