An efficient recombination system for chromosome engineering in <i>Escherichia coli</i>

Yu, Daiguan; Ellis, Hilary; Lee, E-Chiang; A. Jenkins, Nancy; G. Copeland, Neal; L. Court, Donald

doi:10.1073/pnas.100127597

Public

An efficient recombination system for chromosome engineering in Escherichia coli

Published in Proceedings of the National Academy of Sciences • May 16, 2000

Authors:

Daiguan Yu

Hilary Ellis

E-Chiang Lee

Abstract

A recombination system has been developed for efficient chromosome engineering in Escherichia coli by using electroporated linear DNA. A defective lambda prophage supplies functions that protect and recombine an electroporated linear DNA substrate in the bacterial cell. The use of recombination elim...

Subject

Prophage

Plasmid

Escherichia coli

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