Sensitive PCR-Restriction Fragment Length Polymorphism Assay for Detection and Genotyping ofGiardia duodenalisin Human Feces

Amar, Corinne; H. Dear, Paul; Pedraza‐Díaz, Susana; Looker, Nick; Linnane, Eithne; McLauchlin, Jim

doi:10.1128/jcm.40.2.446-452.2002

Public

Sensitive PCR-Restriction Fragment Length Polymorphism Assay for Detection and Genotyping ofGiardia duodenalisin Human Feces

Published in Journal of Clinical Microbiology • Feb 1, 2002

Authors:

Corinne Amar

Paul H. Dear

Susana Pedraza‐Díaz

Abstract

An assay that uses heminested PCR-restriction fragment length polymorphism analysis for the detection and genotyping of Giardia duodenalis on the basis of polymorphism in the triose phosphate isomerase (tpi) gene was developed. This assay was evaluated with DNA extracted from purified parasite mater...

Subject

Genotyping

Biology

Feces

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