Age-related decrease in TCR repertoire diversity measured with deep and normalized sequence profiling.

V Britanova, Olga; V Putintseva, Ekaterina; Shugay, Mikhail; M Merzlyak, Ekaterina; A Turchaninova, Maria; B Staroverov, Dmitriy; A Bolotin, Dmitriy; Lukyanov, Sergey; A Bogdanova, Ekaterina; Z Mamedov, Ilgar; B Lebedev, Yuriy; M Chudakov, Dmitriy

doi:10.4049/jimmunol.1302064

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Age-related decrease in TCR repertoire diversity measured with deep and normalized sequence profiling.

Shared by NobleBlocks on Mar 15, 2014 • 12:00 AM UTC

Authors:

Olga V Britanova

Ekaterina V Putintseva

Mikhail Shugay

Abstract

The decrease of TCR diversity with aging has never been studied by direct methods. In this study, we combined high-throughput Illumina sequencing with unique cDNA molecular identifier technology to achieve deep and precisely normalized profiling of TCR β repertoires in 39 healthy donors aged 6-90 y....

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