Rapid Method for Species-Specific Identification of <i>Vibrio cholerae</i> Using Primers Targeted to the Gene of Outer Membrane Protein OmpW

Nandi, Bisweswar; K. Nandy, Ranjan; Mukhopadhyay, Sarmishtha; Balakrish Nair, G.; Shimada, Toshio; C. Ghose, Asoke

doi:10.1128/jcm.38.11.4145-4151.2000

Public

Rapid Method for Species-Specific Identification of Vibrio cholerae Using Primers Targeted to the Gene of Outer Membrane Protein OmpW

Published in Journal of Clinical Microbiology • Nov 1, 2000

Authors:

Bisweswar Nandi

Ranjan K. Nandy

Sarmishtha Mukhopadhyay

Abstract

The distribution of genes for an outer membrane protein (OmpW) and a regulatory protein (ToxR) in Vibrio cholerae and other organisms was studied using respective primers and probes. PCR amplification results showed that all (100%) of the 254 V. cholerae strains tested were positive for ompW and 229...

Subject

Biology

Vibrio cholerae

Amplicon

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