CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites

Naito, Yuki; Hino, Kimihiro; Bono, Hidemasa; Ui-Tei, Kumiko

doi:10.1093/bioinformatics/btu743

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CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites

Shared by NobleBlocks on Dec 9, 2014 • 12:00 AM UTC

Authors:

Yuki Naito

Kimihiro Hino

Hidemasa Bono

Abstract

Abstract Summary: CRISPRdirect is a simple and functional web server for selecting rational CRISPR/Cas targets from an input sequence. The CRISPR/Cas system is a promising technique for genome engineering which allows target-specific cleavage of genomic DNA guided by Cas9 nuclease in complex with a ...

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