Gaussian approximations of fluorescence microscope point-spread function models

Zhang, Bo; Zerubia, Josiane; Olivo‐Marín, Jean‐Christophe

doi:10.1364/ao.46.001819

Public

Gaussian approximations of fluorescence microscope point-spread function models

Published in Applied Optics • Mar 13, 2007

Authors:

Bo Zhang

Josiane Zerubia

Jean‐Christophe Olivo‐Marín

Abstract

We comprehensively study the least-squares Gaussian approximations of the diffraction-limited 2D-3D paraxial-nonparaxial point-spread functions (PSFs) of the wide field fluorescence microscope (WFFM), the laser scanning confocal microscope (LSCM), and the disk scanning confocal microscope (DSCM). Th...

Subject

Paraxial approximation

Gaussian

Optics

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