The BRET technology and its application to screening assays

Bacart, Johan; Corbel, Caroline; Jockers, Ralf; Bach, Stéphane; Couturier, Cyril

doi:10.1002/biot.200700222

Public

The BRET technology and its application to screening assays

Published in Biotechnology Journal • Jan 28, 2008

Authors:

Johan Bacart

Caroline Corbel

Ralf Jockers

Abstract

The bioluminescence resonance energy transfer (BRET) method is based on resonance energy transfer between a light-emitting enzyme and a fluorescent acceptor. Since its first description in 1999, several versions of BRET have been described using different substrates and energy donor/acceptor couples...

Subject

Proteases

Förster resonance energy transfer

Energy transfer

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