Quantification of Tetracycline Resistance Genes in Feedlot Lagoons by Real-Time PCR

S. Smith, Marilyn; K. Yang, Richard; W. Knapp, Charles; Niu, Yafen; Peak, Nicholas; M. Hanfelt, Margery; C. Galland, John; W. Graham, David

doi:10.1128/aem.70.12.7372-7377.2004

Public

Quantification of Tetracycline Resistance Genes in Feedlot Lagoons by Real-Time PCR

Published in Applied and Environmental Microbiology • Dec 1, 2004

Authors:

Marilyn S. Smith

Richard K. Yang

Charles W. Knapp

Abstract

A new real-time PCR method is presented that detects and quantifies three tetracycline resistance (Tcr) genes [tet(O), tet(W), and tet(Q)] in mixed microbial communities resident in feedlot lagoon wastewater. Tcr gene real-time TaqMan primer-probe sets were developed and optimized to quantify the Tc...

Subject

Tetracycline

TaqMan

Biology

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