A Nonempirical Method Using LC/MS for Determination of the Absolute Configuration of Constituent Amino Acids in a Peptide:  Combination of Marfey's Method with Mass Spectrometry and Its Practical Application

Fujii, Kiyonaga; Ikai, Yoshitomo; Oka, Hisao; Suzuki, Makoto; Harada, Kenichi

doi:10.1021/ac970289b

Public

A Nonempirical Method Using LC/MS for Determination of the Absolute Configuration of Constituent Amino Acids in a Peptide: Combination of Marfey's Method with Mass Spectrometry and Its Practical Application

Published in Analytical Chemistry • Dec 1, 1997

Authors:

Kiyonaga Fujii

Yoshitomo Ikai

Hisao Oka

Abstract

The “advanced Marfey's method” proposed in our preceding paper has been developed to nonempirically determine the absolute configuration of constituent amino acids in a peptide using liquid chromatography/mass spectrometry (LC/MS). For the establishment of this method, we had to resolve the followin...

Subject

Chemistry

Chromatography

Mass spectrometry

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